In Vitro Testing

Cell Culture Based Testing

Design and develop appropriate in vitro assays to test the toxicity/activity of the drug/inhibitor/polymer.

  • IC50 toxicity analyses, Cell Viability analyses (Annexin V, MTT etc.)

  • DNA/RNA-based genetic assays (RT-PCR, PCR array, NanoString etc.)

  • Western blot and Immunofluorescence-based assays to track the downstream signalling

  • ELISA for the molecule under investigation.

 

In vitro drug loading and release studies

Design of experimental protocols to best mimic in vivo conditions

  • Determining and quantifying drug using HPLC with different detectors including UV, Fluorescence and Mass spectrometer.

  • Incorporation drug into bulk material, micelles, nanoparticles and microemulsions etc.

  • Assessment of the stability of loaded drugs

Available Cell Lines

             Human Primary Retinal Pigment Epithelial Cells (ARPE-19)

             Human Primary Lens Epithelial Cells (FHL-124, HLEB3)

             Human Primary Trabecular Meshwork Cells (HTM)

             Human Primary Corneal Epithelial Cells (HCEC, Transformed)

             Mouse Retinal Ganglion Cells (RGC-5)